ࡱ> 463 RHbjbj7H77777KKKK_ K4k\\\$sB7\\\\\B77W\R77\ .K m0MMM7P\\\BB\\\\\\\M\\\\\\\\\ : Design of Biointerfaces in 2D and 3D for Directing Biological Response of Proteins and CellsMarcus Textor, Prof. emer.ETH Zurich, BioInterfaceGroup, Department of Materials, Zurich, SwitzerlandE-mail:  HYPERLINK "mailto:marcus.textor@mat.ethz.ch" marcus.textor@mat.ethz.chSurface modifications based on biochemical or biological principles are important tools for the fabrication of biosensor chips, biomedical devices such as implants, and of drug delivery carriers. Moreover, well-designed model biointerfaces have substantially contributed in the last decade to a better insight into fundamental aspects of protein-surface and cell-surface interaction. I will provide an overview on tools enabling the surface engineer to tailor the interface of biomaterials and biosensors, with special emphasis on self-assembly approaches of eliminating non-specific adsorption and adding biological functionalities such as peptides, carbohydrates/sugars, proteins/antibodies. I will also address the importance of static ex-situ surface analysis and of dynamic, in-situ techniques to quantitatively monitor the kinetics of adsorption in the context of both the surface modification by self-assembly as well as the determination of (specific) biointeractions.In a second part, I will present results from culturing mammalian cells on 2D patterned surfaces and in microfabricated 3D wells, either hosting single cells or well defined cell clusters. Novel types of 3D culture substrates were produced in PDMS polymers and PEG hydrogels by efficient replication techniques from lithographically produced masters. Cells in 3D microenvironment were found to have very different adhesion, morphology and function. Finally, we have investigated the response of breast cancer cell clusters in 3D PEG hydrogel wells to the drug Taxol*, demonstrating a platform that allows to interrogate separately factors affecting drug response, in particular dimensionality, cell-cell and cell-matrix interaction. Advanced 3D cell culture platforms have a great potential to reduce or eliminate in the future the need for animal testing.]^ktx  m ıćmXE22$hpMh:CJOJQJaJmH sH $hpMh~qCJOJQJaJmH sH (hpMh0JCJOJQJaJmH sH 3jhpMhCJOJQJUaJmH sH -jhpMhCJOJQJUaJmH sH $hpMhdCJOJQJaJmH sH $hpMhaCJOJQJaJmH sH $hpMhCJOJQJaJmH sH 'hpMh5CJOJQJaJmH sH 'hpMhU05CJOJQJaJmH sH ^y  GH $(a$gdpM $d@xa$gd d@xgd$a$ $xa$gd$a$gdU0   ( + /  #)CJFGHŞŞŞ؞~؞m hpMhCJOJQJmH sH hqf{CJOJQJaJmH sH h>.ICJOJQJaJmH sH $hpMhU0CJOJQJaJmH sH 'hpMh6CJOJQJaJmH sH $hpMhCJOJQJaJmH sH $hpMh:CJOJQJaJmH sH 'hpMh:6CJOJQJaJmH sH 501:p}. A!"#$% DyK marcus.textor@mat.ethz.chyK Bmailto:marcus.textor@mat.ethz.chb PJ_HmH nHsH tH4`4 ckeCJ_HmHsHtH VV 4e8h 1$@&gdm\&56OJQJ^J_HaJmH sH tH $A $ ؞k=W[SOJiJ nfh*B*,V , S##]vc>*B* PK![Content_Types].xmlj0Eжr(΢Iw},-j4 wP-t#bΙ{UTU^hd}㨫)*1P' ^W0)T9<l#$yi};~@(Hu* Dנz/0ǰ $ X3aZ,D0j~3߶b~i>3\`?/[G\!-Rk.sԻ..a濭?PK!֧6 _rels/.relsj0 }Q%v/C/}(h"O = C?hv=Ʌ%[xp{۵_Pѣ<1H0ORBdJE4b$q_6LR7`0̞O,En7Lib/SeеPK!kytheme/theme/themeManager.xml M @}w7c(EbˮCAǠҟ7՛KY,e.|,H,lxɴIsQ}#Ր ֵ+!,^$j=GW)E+& 8PK!mQtheme/theme/theme1.xmlYMoE#F{oc'vGuرhF[x=ޝzvg53NjHHzZ $ʯI)*E_N&IA!>Ǽ3xN>UWL0Č߫߿z}rx{h9T8T/χ?xY'~3S~O_|w)ӈHt=a+d NG1-RlƁ1RJwT蠯M1K"o he˓ێ½PL-|%EhY7'qP.\L=dqķ3Iofi.ñBRb-JP_pG ݢiKtdӌhFio7;7Q2ȾD>a/Q>XW ˔M_uHq)h +:Viw4rBqϫ"r!2laCbU|!8^8>ܠ,AбVt׎~ls14_=,ɬožTV G"ѦbH'.4x޵w-ϷE|F;v`b3"G 'e\fHO '$i_wp >*8i&LY%\,xҕ= r6f 3Tʅ):ªZKLs&C Mܛ0 [˫p@ע`j۽7 YHxHicT5Ar@HZAZC}i' RQ\m,zo,gQu{Y\,Nר/=L _.̇Y7C6-fSh62"5NHT[X65̫4X%Yֳ2fkhi~tCKF#b +w1m|4`!:U!p5a:~{4mmio ήc8mDJpSǹ橠V1?#Si?3E'pS2W`qB]( 08w nA[s)k8= Aa?R dڒɾcUӽ˲d)#Queb}C!&i0>4S7{z F} 4sKvUKoȳh~1jYUVH5U8Vk;֜L9ŰD ?*|FL"A3>g]dm2iVֵ褽mg H H H HX8@0( CCCC B S  ?  Ud#JJd:a?I{3>.IpMe^kfqf{~qU0xHJ@GGGGHh@UnknownG*Ax Times New Roman5Symbol3. *Cx Arial;([SOSimSunA BCambria Math AhS'S'BSF< < r4dDDJ#q?qO2FDesign of Biointerfaces at the Micro- and Nanoscale and Application toMarcus TextorUSER-Oh+'0  < H T`lt|HDesign of Biointerfaces at the Micro- and Nanoscale and Application toMarcus TextorNormalUSER-3Microsoft Office Word@G@Oa@ @ < ՜.+,D՜.+,|8 hp   ETH ZrichD HDesign of Biointerfaces at the Micro- and Nanoscale and Application to Title 8@ _PID_HLINKSA|:!mailto:marcus.textor@mat.ethz.ch0   !"$%&'()*,-./0125Root Entry F`.7Data 1TableMWordDocument7SummaryInformation(#DocumentSummaryInformation8+CompObje  F#Microsoft Office Word 97-2003 ĵ MSWordDoc9q